Lab Report - Protein Separation

Biomarker discovery in human breast cancer cells

Lab based Practical

Molecular Pharmacology BHS006-6

Breast cancer is the most common cancer type among women and the ability to identify potential biomarkers or hallmarks of early disease is critical for early detection and treatment. The aim of this lab based practical session is to utilise an established protein separation technique (SDS-PAGE) to identify possible biomarkers, which are present in 100pM Estradiol, treated breast cancer cell homogenates versus control cells.

Students will run protein samples on SDS-PAGE gel as per protocol below to demonstrate protein separation and identify differing bands, between control and treated cells, which may be biomarkers of disease.

Equipment & Materials:

Running buffer [[url removed, login to view] Tris/[url removed, login to view] glycine/0.1% (w/v) SDS, pH8.3]

Pre-stained molecular markers (PageRuler)

Coomassie Blue Stain Solution

Human breast cancer cell homogenates: a) control cells; b) cells treated with 100pM 17β-estradiol

Human recombinant histone proteins: H2B; H3.3 [1μg (1μl) added to 20μl sample buffer]

Experiment Procedures:

Preparation of Sodium Dodecyl Sulphate-Polyacrylamide (SDS-PAGE) gel will be undertaken by laboratory staff prior to the practical. The protocol for the preparation of the gel can be found at the end of this document.

Skills: Research Writing

See more: i want 5 samples of easy editing for grade 7 students, final report lab water quality contamination, formal aspirin report lab, lab report cdma system, write electrical engineering lab report, example matlab lab report, lab report data, wealth lab report, sample medical lab report analysis, project report writting free samples, lab report data analysis, data analysis lab report, project report computer lab management, indesign template lab report, lab report database, samples visio diagrams server racks equipment, report cover design samples, ammeter voltmeter lab report, indesign lab report templates, samples marketing report writing, report writing samples medical projects, free synopsis samples mba project report, companies daily report form samples, project report design samples website hotels, ppc performance report online samples

About the Employer:
( 9 reviews ) London, United Kingdom

Project ID: #12179151

13 freelancers are bidding on average $179 for this job


I'm a PhD student in Engineering with a Master's in Statistics. Expert in Matlab, Minitab Econometric and statistical modeling of pretty much everything you can think of. These include expertise on protocol development More

$170 USD in 1 day
(575 Reviews)

Hi, I would like to use my experience to serve you better. You can trust my sincere indulgence both in quality and time frame. If you will give me a chance, I will ensure that you're satisfied. I'm really interested in More

$150 USD in 5 days
(300 Reviews)

I am a competent academic writer, with high lead times and turnaround time, proposal writer, masters level dissertation writer, PHD level dissertation and thesis writer, PHD defense PowerPoint presentation writer, argu More

$132 USD in 1 day
(289 Reviews)

Hello I'm creative with superb writing skills and a passion for the job. I don't just get over projects but I always effort to deliver the best as per clients' requirements and leave them satisfied. Kindly give me more More

$150 USD in 3 days
(76 Reviews)

Hi I am Msc in molecular biology, medical biotechnology and microbiology. I have 7 years of research and writing experience and have worked on PhD level thesis projects, published papers in peer reviewed journals and h More

$150 USD in 7 days
(35 Reviews)
$216 USD in 2 days
(72 Reviews)

Hello, how are you doing today? Click on that__CHAT__button below so that I can ask a few questions concerning your project. I have read all the provided instructions and I am the right person to work on this task. I p More

$100 USD in 1 day
(28 Reviews)

Being an academician, I always place bids for academic writing only. I think, I’ll be the apt person to handle your project. Currently, I am a doctoral researcher at the Arizona State University. Being in academics fo More

$100 USD in 3 days
(13 Reviews)

Hello, I have 3 years experience in Articles/Content/Academic writing and assure you that I will provide the quality work on time as per your requirements. I will provide plagiarism free content within the deadlines a More

$24 USD in 1 day
(15 Reviews)

I have majored in Biochemistry and the lab experiment you are talking about was also one of the experimentation that we did. I have an in-depth knowledge and can surely be of help if you give me an opportunity.

$66 USD in 3 days
(14 Reviews)

When it comes to reports, I can provide you with detailed accuracy that stands out. I don’t just start writing about a topic. I take the time to investigate and obtain accurate details because research is imperative. A More

$555 USD in 4 days
(3 Reviews)

If you need articles, you need an experienced writer who can incorporate impeccable grammar, exemplary content, and enticing phrases to create an interesting and informative piece that draws in your target audience. If More

$500 USD in 6 days
(1 Review)

Dear employer, My minor is Biology, and I remember performing similar experiments with SD-PAGE before. I will write the lab report for you as you wished, but you need to provide me the data and results first. I belie More

$15 USD in 0 days
(1 Review)